FORM OF NITROGEN AFFECTS GROWTH OF CATHARANTHUS ROSEUS

5-Methyltryptophan resistant cells of Catharanthus roseus.

Several cell lines resistant to 5-methyltryptophan were selected from wild type cells of different Catharanthus roseus suspension cultures. The resistant cells had up to 30 times the normal levels of free tryptophan. Despite the increased pool size of tryptophan anthranilate synthetase activity of resistant cells was as sensitive to inhibition by L-tryptophan as wild type cells. The overproduct...

The seco-iridoid pathway from Catharanthus roseus

The (seco)iridoids and their derivatives, the monoterpenoid indole alkaloids (MIAs), form two large families of plant-derived bioactive compounds with a wide spectrum of high-value pharmacological and insect-repellent activities. Vinblastine and vincristine, MIAs used as anticancer drugs, are produced by Catharanthus roseus in extremely low levels, leading to high market prices and poor availab...

Growth and nitrogen content of sunflower as affected by the rate and form of nitrogen

Cryopreservation of Alkaloid-Producing Cell Cultures of Periwinkle (Catharanthus roseus).

A procedure for cryogenic storage of alkaloid producing cell lines of periwinkle, Catharanthus roseus (L.) G. Don., has been developed. The procedure differs from established cryopreservation protocols in several aspects. Specifically, 4-day-old suspension subcultures of three cell lines were precultured in nutrient media supplemented with 1 molar sorbitol for 6 to 20 hours. The cells were then...

Changes in Fructose-2,6-bisphosphate Level during the Growth of Suspension Cultured Cells of Catharanthus roseus*

Fructose-2,6-bisphosphate (F2,6BP) was discov­ ered as a potent stimulator of phosphofructokinase by van Schaftingen et al. [1]. Other findings indicate that F 2 ,6BP also modulates the activity of some plant enzymes, including pyrophosphate dependent phosphofructokinase (PPi-PFKase) and fructose-1,6bisphosphatase [2]. Since F2,6BP was demonstrated in mung bean seedlings [3], the levels of F2,6...